IBRH3AU Core Facilities & Infrastructure

Immunology Laboratory

Makerere University College of Health Sciences has a state of the art research laboratory on the first floor of the Clinical Research Building outfitted with modern equipment. Its clinical capabilities include testing of HIV, Hepatitis B, Herpes Simplex 2, and syphilis, Pregnancy etc. Research capabilities include PBMC isolation and cryopreservation, cell culture, interferon gamma release assays (IGRA), Whole Blood and Interleukin Assays involving cell processing for intra cellular cytokine assays, T-Spot among others. The laboratory policy is to maintain at least four IATA certified staff to handle specimen shipments. It also provides quality laboratory services to several external projects and research clinics particularly sample processing, storage and shipment.

Staff - The Laboratory team has nine well-trained staff (Director, six technologists, one Manager and a Data Manager) led by the Laboratory director, Assoc. Prof Moses Joloba. The laboratory policy is to maintain at least four IATA certified staff to handle specimen shipments. In addition, the Immunology Laboratory has been an occupational trainer to many students in a large range of immunological research areas. Steve Cose PhD, a resident Immunology consultant, is available for consultation and technical supervision of immunology research techniques ongoing in the Laboratory. All staff are Human Subject Protection trained(HSP) and Good Clinical Laboratory Practice (GCLP) trained.

Read More

The Molecular Biology laboratory

This laboratory was established with support from NIH and SIDA (Sweden). It is approx. 800 sq. ft., air conditioned well equipped and appropriately organized for most molecular biology oriented studies. The laboratory has acquired an additional 2500 sq. ft. space (with offices and a seminar room) that will house the human genetics laboratory. Equipment and software: The laboratory has a real time thermocycler (Rotor Gene Q 6000), two incubator shakers (Innova 3100), incubator (TwinCubator) , hybridization oven (Labnet), four refrigerators, microcentrifuge (Eppendorf), belly dancer (Stovall), electroporator (BioRad), conventional thermocyler (Techne TC-412), three gel electrophoresis systems (), two UV transilluminators, a polaroid camera (UVP), gel imaging system (BioDoc-It), a blotting apparatus, incubator (Binder), pH meter (Oakton), analytical balance (Sartorius), autoclave (Priorclave), an ice maker, sterilizing hood (Cleanspot), crosslinker (Spectrolinker), spectrophotometers (GeneQuant, Cecil, Janeway 3605), five internet linked computers, and a dark room. Software are installed and operational including the Bionumerics for molecular analyses.

Read More

The Molecular Diagnostic Laboratory

The space is located on the top floor of the Pathology/ Microbiology building that includes 225 sq/ft for the pre- amplification room, 240 sq/ft for the amplification laboratory, 340 sq/ft for the post-amplification room, 120 sq/ft for office space and 200 sq/ft for the boardroom with video conferencing facilities installed and functional. Equipment available in this facility include Rotor Gene real-time PCR cycler, spectrophotometer (NanoDrop 2000c), DNA extraction machine (QIAcube), QIAxcel robotic PCR setup (liquid handling), Pipetteman, electrophoretic equipment (BIORAD), hybridization oven (ProBot), -80C freezers (Brunswick), fridges and automated electrophoretic system (QIAgility). Freezing space - currently we have 2 -80C freezers chest and upright with capacity to hold 50,400 and 30,000 samples (in 1.5 - 2mL cryovials) respectively. We have 2 -20C freezers chest and upright with capacity to hold 23,300 and 22,600 samples (in 1.5 - 2mL cryovials) respectively. Approximately one third of this total freezer space is available for the feasibility study. Offices are equipped with computers installed with up-to-date software used for analysis.

Read More

Mycobacteriology (BSL-III) Laboratory

The PI, Prof. Joloba directs this Biosafety Level III (BSL-III) TB culture facility operating since June 2009; with controlled uni-directional flow of HEPA filtered air. It has a level II section and a containment (Level III) section, two office rooms, a reception area, a well-monitored store and a training room. The facility is approximately 90 square meters overall in size and is well monitored with a 24-hour, 7-day per week surveillance system (intelligent Video Management system) for the BSL-II and BSL-III sections. It has separate, well-equipped and operational Immunology and Molecular Biology sections. The BSL-III facility has a dedicated facility manager with 8 years TB-related experience as well as 10 additional BSc level technologists, an MSc level data manager, a quality assurance officer, safety officer, laboratory training coordinator and additional facilities assistants. All personnel are up-to-date with advances in mycobacteriology/molecular and immunology assays as well as quality systems management aspects.

Read More

Liquid Nitrogen plant

Makerere University College of Health Sciences has a liquid nitrogen plant located on site. It has two StirLIN-1 Compact that produce 240 l/day on site LN2 supply. Has power consumption of 16 kW, connected to a 3 phase power supply at 200 to 480 V, 50/60 Hz, cooling water consumption of 1000l/h at 15C located in adequate space. It has a noise level 70 dBA as is environmentally friendly. Plant room temperature is maintained at 25C, altitude 250m, relative humidity 95%, and cooling water temperature 15C. Has a liquid nitrogen storage capacity of 300 l, with a level indication and control.

It has a dedicated generator set for power supply back up, connected to voltage stabilizer for utility power stabilization. The personnel posses liquid nitrogen handling personal protection materials and clothing. The plant meets European CE safety standard, IP54, IEC 60204 and other international standards. It has an integrated system diagnostics, fully automatic start/stop and restart after power failures, no defrosting or purging required, 10 minutes start-up time to full production, single switch operation.

Proposed IBRH3AU Infrastructure Upgrade

The MakCHS has dedicated up to 6500 sq. ft. of space for alterations and renovations to provide adequate infrastructure of a world-class biorepository in Africa.

Space - up to 6500 sq. ft. of space has been dedicated and for the IBRH3AU.

Specialized rooms - among these are a freezer room, nitrogen freezer room, automated DNA/RNA extraction/PCR-set up laboratory, post-PCR laboratory, live cell preparation and freezing laboratory, blood processing laboratory, office space and computer/database laboratory.

Access - a role-based access control system that restricts access to the facility to authorized personnel only will be installed; we shall install electronic finger print access control each major entry and exit points.

Water purification -we shall install a dedicated RO water purification plant for the IBRH3AU laboratories with capacity of 1000LPH, an ISBER recommendation that repositories maintain separate water purification services.

Read More